The antagonism and its mechanism of liquiritin on toxicity to heart induced by aconitine
Sun Jianning Department of Chinese Herbal Pharmacology
Beijing University of Chinese Medicine
China
Abstract:
AIM Radix Glycytthizae was reported widest used in the formulae which have Radix Aconiti Lateralis Preparata to dispel its toxicity. In this study, the antagonism and its mechanism of liquiritin on toxicity to heart induced by aconitine were investigated. METHODS Regarding the mouse's survival rate and rat’s electrocardiogram (ECG) change as the index, the antagonism of liquiritin on toxicity to heart of aconitine in rats was observed. The effects of aconitine and liquiritin on myocardium Voltage-Dependent Na+ channel and L-type Ca2+ channel of guinea pigs through patch clamp technique were observed. RESULTS liquiritin could partly dose-dependent oppose the toxicity of aconitine. The liquiritin 6, 3mg•kg-1 could make the death rate induced by aconitine 0.4 mg•kg-1 reduce by 55%, 30% respectively and significantly. When the dosage of aconitine was 17.5, 42.8, 80.8, 84.5, 106.7µg•kg-1, it appeared in order VEBS, VT, VF, CA and death. The toxicity to heart dosage of aconitine was improved by 0.9%- 930.4% after injection of liquiritin 3, 1.5, 0.75 mg•kg-1 respectively. The liquiritin (10µM) can inhibit the opening of Voltage-Dependent Na+ channel and the L-type calcium ion channel induced by aconitine (1µM), the ratio of restrain was 7.9%, 13.1% respectively. CONCLUSION Liquiritin can antagonize the heart toxicity induced by aconitine. The mechanism of liquiritin on toxicity to heart induced by aconitine is inhibition the Voltage-Dependent Na+ channel and the L-type calcium ion channel.
